jaue2019-070: Screening of Extractant for the Determination of Active Sludge Trehalose in Sewage Treatment

Abstract

This study investigated and proposed the use of trehalose extraction and detection method for the determination 
of active sludge trehalose in sewage treatment. Seven extractants namely; trichloroacetic acid, ethanol, methanol, 
acetone, pure water, formaldehyde and trichloromethane, were used separately to extract the active sludge 
trehalose and their trehalose contents were determined. The results shown in standard curves plotted for all seven 
extractants demonstrated a good linearity, and the regression coefficients varied insignificantly. Using 
trichloroacetic acid, active sludge trehalose was extracted within a period of only 40 min at 40 centigrade. In view 
of that, trichloroacetic acid proved as the most efficient extractants in extracting trehalose from the active sludge. 
Its extraction rate was 4 to 11 times faster than that of the other extractants for the same amount of active sludge. 
From our results, trichloroacetic acid substantiated as the optimal extractant for the determination of active sludge 
trehalose.